Recombinant Human PFK1/PFKM Protein (His & GST Tag)

Size: 50μg

Catalogue Number: PKSH030320-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: PFK1, PFKM

Target Synonym: 6-phosphofructokinase, muscle type, Phosphofructo-1-kinase isozyme A, Phosphofructokinase 1, Phosphohexokinase, PFKM, PFKX, ATP-PFK, GSD7, PFK-1, PFK1, PFKA, PPP1R122

UNIProt ID: P08237

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Fusion Tag: N-His & GST

Accession: P08237-1

Background: PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1, 6-bisphosphate (through PFK-1), fructose 2, 6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1, 6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.

Sequence: Thr 2-Val 780

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 112.9 kDa

Observed MW: 113 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only