Cleaved-Caspase 4/5 p20 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16485-50, BZ16485-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P49662/P51878

Host: Rabbit

Reactivity: Human

Applications: WB,ELISA

All Applications: WB: 1/500-1/1000 ELISA: 1/10000

Background: CASP4 (caspase 4) encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, caspase 4 induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cleaved-Caspase 4/5 p20 in JurkatLysates using Caspase 4/5 antibody. The lane on the right is blocked with the Caspase 4/5 peptide.

Bioworld Molecular Weight: Calculated MW: 43 kDa; Observed MW: 47,22 kDa

Specificity: IgG

Alternative Name: TX; Mih1; ICH-2; Mih1/TX; ICEREL-II; ICE(rel)II

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 4/5. AA range:221-270

Conjugate: Unconjugated

Modification: Cleaved