Recombinant Mouse Carboxylesterase-2/CES2 Protein (His Tag)(Active)

Size: 50μg

Catalogue Number: PKSM040639-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: Carboxylesterase-2; CES2

Target Symptom: ces2A3

Target Species: Mouse

Expression Host: HEK293 Cells

Application: Enzyme

Fusion Tag: C-His

Accession: NP_663578.1

Background: Carboxylesterase 2 (CES2) is a member of the carboxylesterase family and belongs to the multigene family. Carboxylesterase 2 is responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and beroin. It also serves to hydrolyze long-chain fatty acid esters and thioesters. It is speculated that carboxylesterases may play a role in lipid metabolism and the blood-brain barrier system and together with isform 1, are a serine esterase involved in both drug metabolism and activation. Human carboxylesterase 2 is commonly expressed in tumor tissues and irinotecan, a topoisomerase I inhibitor commonly used in the treatment of many solid tumors.

Activity: Measured by its ability to hydrolyze pnitrophenylacetate. The specific activity is > 90, 000 pmoles/min/μg.

Sequence: Met 1-Lys 557

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 60.4 kDa

Observed MW: 52 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only