FITC Anti-Mouse CD162 Antibody [4RA10]

Size: 100μg

Catalogue Number: E-AB-F1034UC-100

Citations, Manuals and MSDS Available upon request.

Abbreviation: CD162

Target Synonym: P-selectin glycoprotein ligand 1, Selplg, PSGL-1, Selectin P ligand, CD162,

Conjugation: FITC

Host: Rat

Species Reactivity: Mouse

Application: FCM

Usage: Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10^6 cells in 100 μL volume].

Isotype: Rat IgG1, κ

Clone No.: 4RA10

UNIProt ID: Q62170

Background: The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.

Concentration: 0.5 mg/mL

Control Catalogue Number: E-AB-F09823C

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

GeneID: 20345

Storage: This product can be stored at 2-8°C for 12 months. Please protect from prolonged exposure to light and do not freeze.

Shipping: Ice bag