SARS-CoV-2 Neutralization Antibody ELISA Kit

Size: 96T

Catalogue Number: E-EL-E608-96T, E-EL-E607-96T, E-EL-E606-96T

Citations, Manuals and MSDS Available upon request.

Abbreviation: SARS-CoV-2 Neutralization Antibody

Target Synonyms: COVID 19 Neutralization Antibody; Anti-RBD; RBD Antibody; S1RBD Antibody;

Research Areas: SARS-CoV-2, COVID 19

Target Species: Human

Application: ELISA

Usage: This ELISA kit applies to the in vitro Qualitative determination of SARS-CoV-2 Neutralization Antibody in serum or plasma.

Tested Sample Types: Serum, Plasma

Type: Competitive

Assay Time: 1.5h

Detection Type: Colormetric

Test Principle: This ELISA kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. _x000D_ The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD). After 37℃ incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.

Cross Reactivity: This kit recognizes Human SARS-CoV-2 Neutralization Antibody in samples. No significant cross-reactivity or interference between Human SARS-CoV-2 Neutralization Antibody and analogues was observed

Recovery Rate: 80%-120%

Precision: Both intra-CV and inter-CV are < 15%.

Storage: 2-8℃, 12 months

Shipping: ice bag