Recombinant Rat Epidermal Growth Factor (rRtEGF)

Catalogue Numbers: PR3001-20, PR3001-100, PR3001-1000

Sizes: 20µg, 100µg, 1.0mg (Contact us for the 1.0mg option)

Source: Escherichia coli

Molecular Weight:6.2 kDa, a single non-glycosylated polypeptide chain containing 54 amino acids, including 3 intramolecular disulfide-bonds.

Purity: 97% by SDS-PAGE and HPLC analyses.

Biological Activity: Fully biologically active when compared to standard. The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.

AA Sequence: MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

Endotoxin: Less than 1EU/mg of rRtEGF as determined by LAL method.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Usage: This material is offered by USA Bioworld biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. Made in China

EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor).